Part:BBa_K4806208:Design

CYP2D6 gene for expression in the chloroplast for Chlamydomonas reinhardtii (Phytobrick)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1280
  Illegal PstI site found at 2636
  Illegal PstI site found at 3069
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1280
  Illegal PstI site found at 2636
  Illegal PstI site found at 3069
  Illegal NotI site found at 1786
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1280
  Illegal PstI site found at 2636
  Illegal PstI site found at 3069
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1280
  Illegal PstI site found at 2636
  Illegal PstI site found at 3069
  Illegal NgoMIV site found at 1923
  Illegal NgoMIV site found at 2272
  Illegal NgoMIV site found at 3613
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has been codon optimized for Chlamydomonas reinhardtii and it is compatible with Phytobricks/MoClo parts.

Source

This composite part is a combination of the following parts:

• 1. PSAD(i)-promotor (A1-B1) (BBa_K4806010)
• 1. CTPPSAD-transit peptide (B2) (BBa_K4806014)
• 2. CYP2D6 gene (B3-B4) (BBa_K4806001)
• 3. HA-tag (B5) (BBa_K3002017)^*
• 4. tRPL23-terminator (B6-C1) (BBa_K3002006)^*

Additionally, connectors were used to create the MoClo overhangs:

• 1. Connector B1-B2 (BBa_K3002302)^*
• 2. Connector B2-B3 (BBa_K3002303)^*
• 3. Connector B4-B5 (BBa_K3002304)^*
• 4. Connector B5-B6 (BBa_K3002305)^*

The ^* marked parts were not created by us. Our results can be found on the experience page of each part.